Upregulation of Nei-Like DNA Glycosylase 3 Predicts Poor Prognosis in Hepatocellular Carcinoma

Figure 1

Overexpression of NEIL3 in HCC tissues. (a) A heatmap showed differential expression of genes in seven pair of HCC by mRNA microarray detection. (b) qRT-PCR revealed that the expression of NEIL3 was significantly higher in tumor tissues than that in corresponding adjacent tissues (n = 12). (c) NEIL3 protein level was detected in 130 pairs of specimens by immunohistochemistry. (d) Histopathological score of immunohistochemistry showed that NEIL3 protein levels were remarkably upregulated in tumor tissues compared with nontumor tissues. (e) Data from the Cancer Genome Atlas database revealed that the mRNA expression level of NEIL3 was significantly upregulated in HCC compared with that in normal tissues (adapted from UALCAN (http://ualcan.path.uab.edu/index.html)). (f) qRT-PCR revealed that NEIL3 mRNA expression was significantly upregulated in seven HCC cell lines compared with the normal hepatic cell line L02. (g) Western blotting revealed that the NEIL3 protein level was significantly upregulated in seven HCC cell lines compared with the normal hepatic cell line L02. NEIL3, Nei-like DNA glycosylase 3; HCC, hepatocellular carcinoma; qRT-PCR, quantitative real-time polymerase chain reaction. and .