P4HA2 Promotes Epithelial-to-Mesenchymal Transition and Glioma Malignancy through the Collagen-Dependent PI3K/AKT Pathway

Figure 2

Knockdown of P4HA2 inhibits proliferation, invasion, and migration of glioma cells in vitro. (a) P4HA2 expression level was detected by western blot in several established GBM cell lines (left). The efficiency of two P4HA2 shRNAs was validated by western blot in U251 and U87MG cells (right). (b) Cell proliferation was detected by wWST-1 assay at 0, 24, 48, and 72 h in U251 and U87MG cells; . (c) Cell invasion of both cell lines was assessed in invasion chamber by crystal violet staining (left, scale bar = 100 μm); the invaded cell number was shown in histogram on the right (). (d) Cell migration was observed and photographed at 48 h (left). Data were presented as the percentage of gap distance at 0 h against 48 h and shown in histograms (right, ).

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