P4HA2 Promotes Epithelial-to-Mesenchymal Transition and Glioma Malignancy through the Collagen-Dependent PI3K/AKT Pathway

Figure 5

Knockdown of P4HA2 suppressed the gene expressions of collagens and the PI3K/AKT pathway. (a) Bubble chart of KEGG pathway analysis figured out the enriched pathways associated with P4HA2 expression, of which the ECM-receptor interaction and PI3K/AKT pathway bear the most significance. (b–d) Immunoblotting assay showed the regulation of three representative collagen types I, III, and IV by P4HA2 knockdown; , compared to control. (e–g) The effect of P4HA2 depletion and enzyme inhibition on the PI3K/AKT signaling pathway was determined by immunoblotting assay in two glioma cell lines; , between sh-P4HA2 and control; , between DHB and control.

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