P4HA2 Promotes Epithelial-to-Mesenchymal Transition and Glioma Malignancy through the Collagen-Dependent PI3K/AKT Pathway

Figure 6

AKT activation partially reversed the antioncogenic effect of P4HA2 knockdown. The inhibitory effect on glioma proliferation (a), cell invasion (b), and migration (c) by P4HA2 depletion was partially reversed by AKT phosphorylation activator SC79; , , . (d, e) Immunoblotting assay showed the reversed expression of previously downregulated EMT-related factors by SC79; ∗, between sh-P4HA2 and control; , between SC79 and sh-P4HA2. (f) A sketch map showing the working model of the mechanism for the oncogenic effect of P4HA2. HIF1α-induced P4HA2 promotes the malignancy of glioma cells by regulating collagen deposition and the downstream PI3K/AKT pathway, which could be targeted by DHB and SC79, respectively. P: phosphorylation; H: hydroxylation.

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