SPP1 Regulates Radiotherapy Sensitivity of Gastric Adenocarcinoma via the Wnt/Beta-Catenin Pathway

Figure 2

Suppression of SPP1 expression affected DNA damage, invasion, and apoptosis of GAC cells. (a) The western blot results verified that SPP1 was overexpressed in AGS and SGC7901 cells compared with control GES-1 cells. (b) The transfected efficiency of si-SPP1 in GAC cells. (c) The phosphorylation of H2AX in AGS and SGC-7901 cells was measured by western blot. (d) The invasiveness in AGS and SGC-7901 cells was measured by transwell assay. (e) The apoptosis ratio in AGS and SGC-7901 cells was measured by flow cytometry. (f) The cell cycle in AGS and SGC-7901 cells was measured by flow cytometry. (g) The apoptosis-related protein in AGS and SGC-7901 cells was measured by western blot.

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