Research Article

The lncRNA B3GALT5-AS1 Functions as an HCC Suppressor by Regulating the miR-934/UFM1 Axis

Figure 7

UFM1 was identified as a target of miR-934. (a) Wild-type and mutant-type display of UFM1 3′UTR and miR-934 binding sequence. (b) Luciferase assays of HCC cells cotransfected with the pGL3-UFM1-wt or pGL3-UFM1-mut and miR-NC or miR-934. The assay showed the inhibitory effect of miR-934 on pGL3-UFM1-wt luciferase activity in HCC cells. (c) UFM1 expression in adjacent tissue and tumor tissue measured by western blot analysis. The UFM1 expression in tumor tissues was significantly lower than that in adjacent tissues. .
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