The lncRNA B3GALT5-AS1 Functions as an HCC Suppressor by Regulating the miR-934/UFM1 Axis

Figure 9

B3GALT5-AS1 inhibits the PI3K/AKT pathway through the UFM1. Western blot analysis of UFM1, p-PI3K, t-PI3K p-AKT, t-AKT, Bcl-2, Bax, Vimentin, and E-cadherin in cells cotransfected with NC or B3GALT5-AS1 ov and si-NC or si-B3GALT5-AS1. B3GALT5-AS1 ov induced by UFM1 resulted in downregulation of p-AKT and p-PI3K and the apoptosis pathway-related proteins Bcl-2 downregulation and Bax upregulation. Meanwhile, EMT pathway-related proteins E-cadherin upregulation and Vimentin downregulation were also observed. B3GALT5-AS1 silencing was the opposite of the above.