Metformin Inhibits the Development of Hypopharyngeal Squamous Cell Carcinoma through Circ_0003214-Mediated MiR-489-3p-ADAM10 Pathway

Figure 5

ADAM10 was a target of miR-489-3p. (a) ADAM10 was predicted as a target of miR-489-3p by starBase v3.0. (b, c)) The predicted target relationship between miR-489-3p and ADAM10 was validated by a dual-luciferase reporter assay. (d, e)) The expression of ADAM10 in HSCC tumor tissues and normal tissues was detected by qRT-PCR and western blot. (f, g)) The expression of ADAM10 in metformin-treated FaDu and Detroit 562 cells was detected by qRT-PCR and western blot. (h) The efficiency of miR-489-3p inhibitor was checked using qRT-PCR. (i, j)) The expression of ADAM10 in metformin-treated FaDu and Detroit 562 cells transfected with anti-miR-489-3p, anti-miR-NC, anti-miR-489-3p + si-ADAM10, or anti-miR-489-3p + si-NC was detected by qRT-PCR and western blot. .

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