circDENND4C Promotes Proliferation and Metastasis of Lung Cancer by Upregulating BRD4 Signaling Pathway

Figure 5

circDENND4C promotes cell growth and invasion by targeting the miR-141-3p/BRD4 axis. (a) The BRD4 gene is determined as a potential target gene of miR-141-3p by TargetScan analysis. (b) qRT-PCR detects the relative expression of BRD4 in the collected NSCLC tissues. (c) Immunohistochemical test further confirms the expression of BRD4 in NSCLC specimens. (d) Pearson correlation analysis of the correlation between BRD4 and miR-141-3p. (e) Luciferase method to study the direct interaction between BRD4 and miR-141-3p. (f) After transfection of miR-141-3p mimic or inhibitor in NSCLC cells, the expression of BRD4 mRNA was detected by PCR. (g) Pearson correlation analysis of the correlation between BRD4 and circDENND4C. (h) PCR was used to detect BRD4 mRNA expression in sh-Ctrl group and sh-circDENND4C group. ^∗P < 0.05 and ^∗∗P < 0.01.