miR-193a Directly Targets PSEN1 and Inhibits Gastric Cancer Cell Growth, the Activation of PI3K/Akt Signaling Pathway, and the Epithelial-to-Mesenchymal Transition

Figure 5

miR-193a regulates PSEN1 expression through binding to PSEN1 mRNA 3′-UTR. (a) PSEN1 was a target gene of miR-193a, and the binding site was located at the 3′-UTR of PSEN1 mRNA. (b) Luciferase reporter assay elucidated miR-193a and decreased the luciferase activity of HGC-27 cells transfected with wild-type PSEN1 3′-UTR, while it did not alter the luciferase activity of cells transfected with mutant PSEN1 3′-UTR. (c) Overexpression of miR-193a inhibited the mRNA level of PSEN1, while knockdown of miR-193a enhanced the expression of PSEN1 in HGC-27 cells. ; ^#.

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