DOK7 Inhibits Cell Proliferation, Migration, and Invasion of Breast Cancer via the PI3K/PTEN/AKT Pathway

Figure 3

Overexpression of DOK7 inhibits proliferation, migration, and invasion in breast cancer cells. (a) The levels of DOK7 in a human noncancerous cell line (MCF-10A) and various breast cancer cell lines (MCF-7, SUM-1315, BT474, HCC-1937, T47D, SKBR3, MDA-MB-231) were analyzed using qRT-PCR assay. The expressions of DOK7 levels were normalized to MCF-10A normal mammary epithelial cells. (b) MDA-MB-231 and SKBR3 cells were transfected with DOK7-overexpressed or controlled plasmids, and the mRNA levels of DOK7 were evaluated using qRT-PCR assay. (c) The protein levels of DOK7 were analyzed using western blotting assay. (d) Cell viability in vector groups and DOK7 overexpression groups was analyzed using CCK8 assay. (e) Migration assay in the vector group and DOK7 group was performed using transwell assay without Matrigel gel. (f) Invasion assay in the vector group and DOK7 group was performed using transwell assay with Matrigel gel.  < 0.05;  < 0.01;  < 0.001; and  < 0.0001.

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