DOK7 Inhibits Cell Proliferation, Migration, and Invasion of Breast Cancer via the PI3K/PTEN/AKT Pathway

Figure 4

Knocking down DOK7 promotes proliferation, migration, and invasion in breast cancer cells. (a) MCF-7 and SUM-1315 cells were transfected with DOK7 shRNA or scrambled control-shRNA and the qRT-PCR assay was used to measure the mRNA levels of DOK7. (b) Western blotting assay was used to check the protein levels of DOK7. (c) Cell viability in the nc-shRNA group (cells transfected with scrambled control-shRNA) and shDOK7 group (cells transfected with DOK7 shRNA) was analyzed using CCK8 assay. (d) Migration assay in the nc-shRNA group and shDOK7 group was performed using transwell assay without Matrigel gel. (e) Invasion assay in the nc-shRNA group and shDOK7 group was performed using transwell assay with Matrigel gel.  < 0.05;  < 0.001.

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