DOK7 Inhibits Cell Proliferation, Migration, and Invasion of Breast Cancer via the PI3K/PTEN/AKT Pathway

Figure 6

DOK7 inhibits proliferation, migration, and invasion of breast cancer via the PI3K/PTEN/AKT pathway. (a) Western blotting assay was performed on PI3K/PTEN/AKT pathway-related proteins (AKT, p-AKT, PTEN). (b) Knocking down PTEN expression by siRNAs in MDA-MB-231 and SKBR3 cells was detected using western blotting assay. (c) Cell viability in the vector group, DOK7 group, DOK7/siPTEN group (overexpression DOK7 cells were transfected with PTEN siRNA), and DOK7/SF1670 group (overexpression DOK7 cells were treated with 10 µM SF1670 for 24 h) were analyzed using CCK8 assay. Cells in vector group, DOK7 group, DOK7/siPTEN group, and DOK7/SF1670 group were performed using (d) migration and (e) invasion assays. (f)–(h) The tumor sizes and tumor weights in vector, DOK7, and DOK7/SF1670 groups were calculated.  < 0.05;  < 0.01; ns, no significant.

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