circ_0075943 Dominates the miR-141-3p/AK2 Network to Support the Development of Breast Carcinoma

Figure 4

Repression of miR-141-3p reversed the influence on circ_0075943 dwindling. In the rescue experiment, BT-474 and MCF-7 cells were infected with siRNA-circ_0075943 or siRNA-circ_0075943+anti-miR-141-3p, and siRNA-NC or siRNA-circ_0075943+anti-miR-NC was adopted as control. (a) Real-time PCR checks the miR-141-3p level in these cells. (b, c) MTT method was adopted to check cell growth. (d) Detection of cell multiplication by colony formation test. (e and f) Wound healing test and Transwell test were adopted to evaluate cell migration. (g) The Transwell method checks cell invasion. (h) Flow cytometry to check cell apoptosis. (i, j) The immunoblotting technique was adopted to check protein expression. , , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)