circ_0075943 Dominates the miR-141-3p/AK2 Network to Support the Development of Breast Carcinoma

Figure 6

Recovery of miR-141-3p suppresses BC cell phenotype by degrading AK2. Transfect miR-141-3p or miR-141-3p + AK2 in BC cells, and conduct rescue experiments with miR-NC or miR-141-3p + vector as a control. (a) The immunoblotting technique checks AK2 level protein in MCF-7 and BT-474 cells. (b, c) MTT method was adopted to check cell growth. (d) The colony formation test observes cell multiplication. (e, f) Evaluation of cell migration by Transwell and wound healing test. (g) The Transwell experiment evaluates cell invasion potential. (h) Flow cytometry to check cell apoptosis. (i, j) Flow cytometry to monitor cell cycle progression. (k, l) The immunoblotting technique checked Vimentin and c-myc protein levels. , , .

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