ARHGAP11A Promotes the Malignant Progression of Gastric Cancer by Regulating the Stability of Actin Filaments through TPM1

Figure 2

The effect of ARHGAP11A on the proliferation of gastric cancer cells in vivo and in vitro. (a) Western blot verified the ARHGAP11A knockout efficiency. (b) Through continuous observation and counting of gastric cancer cells. It was found that the proliferation rate of ARHGAP11A knockout cells was significantly reduced. (c) Cell proliferation ability was evaluated by an EdU incorporation assay, and it was found that the cell proliferation ability of gastric cancer cells with ARHGAP11A knockout was reduced. Scale bar: 100 μm. ((d) and (e)) Colony formation experiments showed that the proliferation ability of ARHGAP11A knockout cells was reduced. (f) Subcutaneous tumor growth in nude mice in the control group and ARHGAP11A knockout group. (g) Tumor volumes were measured every other day. (h) Tumor weights in the two groups. . (i) Representative images of tumor HE staining and Ki-67 and ARHGAP11A immunohistochemistry staining. Scale bar: 200 μm.

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