Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation

Figure 5

Constitutively active AKT (CA-AKT) significantly reverses the inhibitory effect of ABZ treatment on the migration and invasion of melanoma cells. (a-b) The CA-AKT plasmids were transiently transfected into A375 and B16-F10 cells, while the control group was transfected with the pcDNA3.1 (+) vector only. At 24 h after treatment with 0.4 μM ABZ, western blot analysis was used to detect the expression levels of N-cadherin, E-cadherin, pAKT, pGSK-3β (Ser9/Tyr216), and Snail in the cytoplasm of A375 and B16-F10 cells. The histograms (bottom) show the relative density of the detected proteins. (c-d) The wound healing area and relative wound closure rate (%) in melanoma cells were quantified and analyzed 24 h after treatment with 0.4 μM ABZ. The histograms (bottom) show the relative wound closure rate for each group. (e-f) Results of the transwell invasion experiments for A375 and B16-F10 cells under different conditions. The histograms (bottom) show the number of invasive cells in each group. The data are expressed as means ± SD. All experiments were performed thrice. ; ; .

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