lncRNA MAGI2-AS3 Exerts Antioncogenic Roles in Hepatocellular Carcinoma via Regulating the miR-519c-3p/TXNIP Axis

Figure 6

TXNIP was direct target of miR-519c-3p. (a) Binding region between TXNIP and miR-519c-3p predicted by TargetScan. (b) Luciferase assay showing miR-519c-3p mimic decreased luciferase activity of TXNIP-WT but not TXNIP-MT. (c) RIP assay showed the coenrichment of MAGI2-AS3, TXNIP, and miR-519c-3p. (d) MAGI2-AS3 and TXNIP expression in complex pulled down by biotin-miR-519c-3p-WT or biotin-miR-519c-3p-MT. (e) MAGI2-AS3 expression in HCC tissues and normal tissues analyzed at ENCORI. (f) MAGI2-AS3 expression in HCC tissues and normal tissues analyzed by RT-qPCR. (g) MAGI2-AS3 expression in HCC cells and normal cells analyzed by RT-qPCR. (h) Correlation of MAGI2-AS3 and miR-519c-3p analyzed at ENCORI. HCC, hepatocellular carcinoma; MAGI2-AS3, MAGI2 antisense RNA 3; RT-qPCR, real-time quantitative PCR; WT, wild type; MT, mutant; RIP, RNA immunoprecipitation; miR-519c-3p, microRNA-519c-3p; miR-con, negative control miRNA; TXNIP, thioredoxin interacting protein.

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