CWHM-1008 Induces Apoptosis and Protective Autophagy through the Akt/mTOR Axis in LUAD Cells

Figure 1

CWHM-1008 inhibits cell proliferation in human lung adenocarcinoma (LUAD) cells. (a) Chemical structure of CWHM-1008. (b) CWHM-1008 inhibits LUAD cell viability, evaluated using the Cell Counting Kit-8 assay in H358, A549, H-1299, H-827, and Beas-2b cells, treated with different concentrations of CWHM-1008 (0, 5, 10, 20, 40, or 80 µM) for 24 h Data was compared with control (0 µM) in each group. (c) The cell viabilities of H358 (up) and A549 (bottom) cells treated with different concentrations of CWHM-1008 for 0, 24, 48, and 72 h were evaluated using the Cell Counting Kit-8 assay. Data was compared with control (0 h in each group. (d) H358 and A549 cells were cultured at different concentrations of CWHM-1008 (0, 2.5, 5, or 10 µM) for 10 days. Then, colony formation for each group was observed with crystal violet staining. Data was compared with control (treated with 0 µM for 10 days) in each group. (e) H358 (left) and A549 (right) cells were cultured at various concentrations of CWHM-1008 (0, 20, 30, or 40 µM) for 0 and 24 h. Cell number was first normalized to control (data at 0 h) in each group, and statistical analysis was performed between groups treated with various concentrations of CWHM-1008 and blank group (treated with 0 µM for 24 h). Data are presented as the mean ± SD, n = 3. , , and ; ns, not significant.

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