CWHM-1008 Induces Apoptosis and Protective Autophagy through the Akt/mTOR Axis in LUAD Cells

Figure 2

CWHM-1008 induces apoptosis in lung adenocarcinoma cells. (a) H358, A549, and Beas-2b cells were cultured at various concentrations of CWHM-1008 (0, 10, 20, 30, or 40 µM) for 24 h. Apoptotic rates of the harvested cells were measured using Annexin V/propidium iodide and detected by flow cytometry. (b) Histograms show the percentages of apoptotic cells. (c) Levels of caspase-3, cleaved-caspase-3, poly-ADP-ribose polymerase (PARP), and cleaved-PARP in H358 and A549 cells cultured at the indicated concentrations of CWHM-1008 for 24 h were detected by western blotting. β-Actin was used as the reference protein. (d) Densitometry quantification of band intensities in C was calculated as shown in the histogram using ImageJ software. Data are presented as the mean ± SD; n = 3; and ; ns, not significant.

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