CWHM-1008 Induces Apoptosis and Protective Autophagy through the Akt/mTOR Axis in LUAD Cells

Figure 4

Suppression of autophagy accelerates the CWHM-1008-induced apoptosis in lung adenocarcinoma cells. (a–d) H358 and A549 cells were treated with the indicated concentrations of CWHM-1008 alone or pretreated with LY294002 (10 µM) for 24 h and then the levels of autophagy-related protein LC3 and P62, and apoptosis-related protein caspase-3 and PARP were analyzed using western blotting. Densitometry quantification of the band intensities in A and C was calculated as shown in the histogram using ImageJ software. (e) Apoptotic rates of H358 (up) and A549 (bottom) cells treated with the indicated concentrations of CWHM-1008 alone or pretreated with LY294002 (10 µM) for 24 h were detected by flow cytometry. (f) Histograms show the percentages of apoptotic cells. Data are presented as the mean ± SD, n = 3. , , and ; ns, not significant.

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