CWHM-1008 Induces Apoptosis and Protective Autophagy through the Akt/mTOR Axis in LUAD Cells

Figure 5

Constitutively active Akt facilitates the CWHM-1008-induced apoptosis in LUAD cells. (a–d) H358 and A549 cells were transfected with 3 µg CA-Akt plasmids or control vector (pcDNA3.1) and then treated with the indicated concentrations of CWHM-1008 for 24 h, and then the levels of autophagy-related protein LC3 and p-Akt, and apoptosis-related protein caspase-3 and PARP were analyzed using western blotting. Densitometry quantification of the band intensities in A and C was calculated as shown in the histogram using ImageJ software. (e) H358 and A549 cells were transfected with 3 µg CA-Akt plasmids or control vector and then treated with the indicated concentrations of CWHM-1008 for 24 h. Then, apoptotic rates of two cells were detected by flow cytometry. (f) Histograms show the percentages of apoptotic cells. Data are presented as the mean ± SD, n = 3. , , and ; ns, not significant.

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