Combining Fluorescent Cell Sorting and Single B Cell Amplification to Screen the Monoclonal Antibody Gene against Human Glypican-1 in Pancreatic Cancer

Figure 1

Cell fluorescent staining and sorting of single cell by flow cytometry. (a) Cell fluorescent staining; NC− means lymphocytes without GPC1-FITC staining. The lymphocytes were sampled from rabbits without immunization. NC+ means lymphocytes stained with GPC1-FITC. The lymphocytes were sampled from rabbits without immunization. GPC1− represents lymphocytes that were not stained with GPC1-FITC from immunized rabbits; GPC1+ denotes lymphocytes that were stained with GPC1-FITC from immunized rabbits. (b) B cell sorting by flow cytometry. GPC1+ cells were all that we want which were sorted by flow cytometry.

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