Combining Fluorescent Cell Sorting and Single B Cell Amplification to Screen the Monoclonal Antibody Gene against Human Glypican-1 in Pancreatic Cancer

Figure 2

Electrophoresis of single-cell amplification. As shown in the electrophoretic gel, lanes 2–15 denote that only one cell sorted was used as the PCR template; lanes 18 and 19 were set as the positive control, where 10 cells sorted were utilized as the PCR template; lanes 20 and 21 were set as the negative control in which no cell was used in PCR. The gene of interest was around 380 bp. Lanes 11, 12, and 14, as well as positive controls, in which the bands of interest can be visibly achieved can be seen.