Combining Fluorescent Cell Sorting and Single B Cell Amplification to Screen the Monoclonal Antibody Gene against Human Glypican-1 in Pancreatic Cancer

Figure 3

Detection of GPC1-12 in transfection supernatant was determined using ELISA and GPC1-12 affinity approaches. (a) Both BSA and medium were set as the negative control; PC (positive control): poly-IgG purified by affinity chromatography from positive rabbit serum. (b) GPC1-12 affinity determination. Enzyme-linked immunosorbent assay (ELISA) was performed to test the binding ability. The initial concentration of the coated antigen was 100 μg/mL, and then it was double diluted. It was shown that compared with antigen and blank control groups, GPC1-12 antibodies purified can specifically bind to GPC1 antigens.

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