Combining Fluorescent Cell Sorting and Single B Cell Amplification to Screen the Monoclonal Antibody Gene against Human Glypican-1 in Pancreatic Cancer

Table 1

Primer name and sequence.

Name	Sequence (5′ to 3′)
TSO^[12]	AAGCAGTGGTATCAACGCAGAGTACrGrG + G^a
Oligo(dT)18	TTTTTTTTTTTTTTTTTT
rIgH-Ld1	CTTCTCCTGGTCGCTGTGCT
rIgH-FR1	CACTCACCTGCACAGYCTCTGGA
rIgK-Ld1	CTGCTGGGGCTCCTGCT
rIgK-FR1	GCTGTGGGAGGCACAGTCAC
rIgL-Ld1-1	CCTCACAGTCCTGGCTCACTGCACA
rIgL-Ld1-2	CCYCCTCCTCKCTCACTGCACA
rIgH-J	TGARGAGAYRGTGACSAGGGT
rIgK-J-1	CGACGACCACCTYGGTCC
rIgK-J-2	TGATTTCCACCTTGGTGCC
rIgL-J	CTGTGACGGTCAGCTKGGTC
rIgHG-C1	GAAGACTGAYGGAGCCTTAGGT
rIgK-C1	GGTGGGAAGATGAGGACAGTAG
rIgL-C1	CCTCTGAGGAGGGCGGRAACA