Exosomal MiR-1290 Promotes Angiogenesis of Hepatocellular Carcinoma via Targeting SMEK1

Figure 4

MiR-1290 targets SMEK1 directly in HUVECs. (a) Flow chart of screening miR-1290 target genes that were associated with angiogenesis. (b) The mRNA levels of 10 candidate genes in HUVECs that were transfected with miR-1290 mimic or NC mimic were detected by qRT-PCR. (c) HUVECs were cotransfected with miR-1290 mimic and the wild-type (WT) 3′UTR constructs of different candidate genes. Luciferase activities were determined 36 h after transfection. Luciferase values were normalized to Renilla activities. (d) The wild-type and mutant miR-1290 binding sites in the predicted target sequences of SMEK1 3′UTR are indicated. (e) HUVECs were cotransfected with miR-1290 mimic and WT or mutant SMEK1 3′UTR constructs. Luciferase activities were determined 36 h after transfection. Luciferase values were normalized to Renilla activities. (f) HUVECs were transfected with different concentrations of miR-1290 mimic for 48 h. The expression of SMEK1 was detected by qRT-PCR and Western blotting. ; ; ; . Ns = not significant.