CircRNF13 Promotes the Malignant Progression of Pancreatic Cancer through Targeting miR-139-5p/IGF1R Axis

Figure 4

The interaction among circRNF13, miR-139-5p, and IGF1R. (a–d) Dual luciferase reporter assay was conducted to verify the combination of circRNF13 with miR-139-5p and miR-139-5p with IGF1R. indicates . The sequence of genes mentioned above and corresponding mutant sequence were shown in (a) and (b). (e) The expression of miR-139-5p in PC cells transfected with miR-139-5p. (f) The expression of IGF1R in PC cell lines transfected with IGF1R overexpressed plasmid. (g) RT-qPCR showed the relative expression of IGF1R in PC cells after transfection of circRNF13 siRNA or cotransfection of circRNF13 and miR-139-5p inhibitor. indicates . (h) Western blot experiment indicated the relative expression of IGF1R, CDK4, CDK6, or cyclinD1 in MIA-PaCa-2 cell transfected with circRNF13 siRNA or miR-139-5p mimics, respectively. Western blot experiment showed the association among circRNF13, miR-139-5p, and IGF1R. (i, j) Alteration of IGF1R CDK4, CDK6, and cyclinD1 expression in MIA-PaCa-2 (i) or PANC-1 (j) after different treatments were analyzed by the western blot experiment.

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