Research Article

Pinin Induces Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma by Regulating m6A Modification

Figure 3

Pinin can interact with METTL3 in HCC. (a) Silver-stained gel image for Mettl3 pulldown assay. (b) Western blot to verify the MS data. (c) Representative image of PLA to detect the in situ interaction of Pinin and Mettl3. (d) Representative image of immunofluorescence colocalization experiments captured by using an N-STORM super-resolution system. (e) Immunofluorescence colocalization experiment for cells with overexpressing Pinin-EGFP and Mettl3-mCherry; the representative images were captured by confocal microscopy. (f-g) Acceptor photobleach-FRET and average fluorescent intensities.
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