Research Article
Pinin Induces Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma by Regulating m6A Modification
Figure 4
Pinin interacts with METTL3 in a phase separation-dependent manner. (a) Graphs plotting intrinsic disorder (PONDR VSL2) for Pinin. (b) The recovery of fluorescence of Pinin puncta after photobleaching. (c) Fluorescence recovery fitted to a curve; dissociation rate constant (koff) and recovery half-time (t1/2) values (mean ± s.d.) were calculated from n = 3 independent experiments. (d) Partial FRAP experiment within a Pinin puncta. Graphs indicate line profiles of fluorescence intensity (FI) in the abovementioned images. (e) The Pinin puncta coalescenced to form one large punctum. (f) FRAP experiment of Pinin after treatment with 1,6-hexanediol (HEX). (g) Graph showing the change in fluorescence intensity. (h) FRET experiment to detect the interaction of METTL3 and Pinin after treatment HEX. (i) Graph showing the change in fluorescence intensity.
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