Downregulation of PART1 Inhibits Proliferation and Differentiation of Hep3B Cells by Targeting hsa-miR-3529-3p/FOXC2 Axis

Figure 4

Effect of PAPT1-hsa-miR-3529-3p on regulating the occurrence and development of liver cancer. (a) PAPT1 is a target of miR-3529-3p. Left: schematic representation of the miR-3529-3p site in the PAPT1 3′-UTR. Right: the 3′-UTR reporter assay was carried out in Hep3B transfected with miR-3529-3p mimic or mimic NC. The MUT or WT reporter plasmids were transfected with Lipo-2000. Luciferase assays were performed 48 h after transfection. Firefly luciferase activity was standardized to a Renilla luciferase control. (b) PAPT1 expression was negatively correlated with hsa-miR-3529-3p expression in liver cancer serums. (c) The cell viability of Hep3B cells including the CCK analysis and clone formation; (d) the transwell invasion assays of Hep3B cells transfected with the shlnc and the miR-3529-3p inhibitor; (e) FOXC2, MMP-2, and MMP-9 protein expression in Hep3B cells. All protein samples were isolated in Hep3B cells after transfection with shlnc and the miR-3529-3p inhibitor. Compared with control group, ; ; .

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