Downregulation of PART1 Inhibits Proliferation and Differentiation of Hep3B Cells by Targeting hsa-miR-3529-3p/FOXC2 Axis

Figure 5

Effect of hsa-miR-3529-3p/FOXC2 axis on regulating the occurrence and development of liver cancer. (a) FOXC2 is a target of miR-3529-3p. Left: Schematic representation of the miR-3529-3p site in the FOXC2 3′-UTR. Right: The 3′-UTR reporter assay was carried out in Hep3B transfected with miR-3529-3p mimic or mimic NC. The MUT or WT reporter plasmids were transfected with Lipo-2000. Luciferase assays were performed 48 h after transfection. Firefly luciferase activity was standardized to a Renilla luciferase control. (b) hsa-miR-3529-3p expression was negatively correlated with FOXC2 expression in liver cancer serums. (c) The cell viability of Hep3B cells including the CCK analysis and clone formation. (d) The transwell invasion assays of Hep3B cells transfected with the miR-3529-3p mimic and the overexpression of FOXC2; (e) FOXC2, MMP-2, and MMP-9 protein expression in Hep3B cells. All protein samples were isolated in Hep3B cells after transfection with the miR-3529-3p mimic and the overexpression of FOXC2. Compared with control group, ; ; .

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