circITGA7 Acts as a miR-370-3p Sponge to Suppress the Proliferation of Prostate Cancer

Figure 4

circITGA7 sponges miR-370-3p in PCa cells. (a) Bioinformatics analysis with miRanda, TargetScan, and CircInteractome. (b) The GEO dataset analysis showed the expression of miR-370-3p in PCa cells and normal prostate epithelial cells. (c) The expression level of miR-370-3p in 28 pairs of PCa and paracancerous tissues was detected. (d) Evaluating the correlation of circITGA7 and miR-370-3p expression in PCa tissues by Pearson correlation coefficient. (e) miR-370-3p was downregulated after the overexpression of circITGA7. (f) qRT-PCR was used to detect the relative expression of miR-370-3p in cells transfected with miR-370-3p mimic or negative control. (g) Schematic representation of the binding sites of miR-370-3p with circITGA7. (h) Relative luciferase activity was determined by dual‐luciferase reporter assay in PCa cells cotransfected with miR-370-3p mimic and WT‐circITGA7 or Mut‐circITGA7. (i, j) Interaction between miR-370-3p and circITGA7 was further confirmed by RIP assay. Data were presented as mean ± SD of three independent experiments. Statistically significant differences were indicated: .

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