LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis

Figure 3

LINC00662 acts as a sponge for miR-16-5p. (a) The subcellular localization of LINCOO662 in U2OS and SAOS‐2 cells was detected using FISH. (b) The possible miRNAs which might bind to LINC00662 were predicted at the starBase website (search category: Pan-Cancer: 10 cancer types). (c) The enrichment of miRNAs in U2OS and SAOS‐2 cells was measured using RNA pull-down followed by RT-qPCR. (d) RT-qPCR was carried out to evaluate the transfection efficiency of miR-16-5p mimics in U2OS and SAOS‐2 cells. (e) The binding site between LINC00662 and miR-16-5p. (f) Luciferase activities of U2OS and SAOS‐2 cells after cotransfection with wild‐type or mutant LINC00662 vectors and miR-16-5p mimics or NC mimics. (g) The expression level of miR-16-5p in OS cell lines (U2OS, SAOS‐2, 143B, and MG63) and normal human line HFOB 1.19 was detected using RT-qPCR. (h) The expression of miR-16-5p in 51 OS tissues and adjacent normal tissues was evaluated by RT-qPCR. (i) The influence of miR-16-5p mimics on LINC00662 expression was examined by RT-qPCR in U2OS and SAOS‐2 cells. ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)