LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis

<div>ITPR1 is directly targeted by miR-16-5p. (a) The possible mRNAs binding to miR-16-5p were predicted by PITA, RNA22, and PicTar databases. (b) The expression of candidate mRNAs in U2OS and SAOS‐2 cells transfected with miR-16-5p mimics was measured by RT-qPCR. (c-d) RT-qPCR was performed to determine the expression of ITPR1 in OS cell lines and tissues. (e) The impact of sh-LINC00662 or miR-16-5p mimics in ITPR1 expression was examined by RT-qPCR. (f) The binding site between miR-16-5p and ITPR1 obtained from starBase. (g) Luciferase reporter assay was used to test the interaction between miR-16-5p and ITPR1. (h) Interaction of miR-16-5p with ITPR1 in U2OS and SAOS‐2 cells was confirmed by RNA pull‐down assay. <span class="nowrap"><svg height="13.9365pt" id="M41" style="vertical-align:-3.834pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 13.9365" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z"></path></g><g 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Journal of Oncology

fig4

Figure 4

Figure 4: LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis 