Advanced Molecular Characterization Using Digital Spatial Profiling Technology on Immunooncology Targets in Methylated Compared with Unmethylated IDH-Wildtype Glioblastoma

Figure 1

Digital Spatial Profiling workflow. Morphology markers (GFAP, DAPI, and CD3) plus a high-plex oligo-labelled antibody cocktail were first applied to the section. Regions of Interest (ROIs) were then selected for high-plex profiling using visible wavelength low-plex imaging to establish the tumour “geography.” Ultraviolet was used to release the oligo tags at the selected ROIs. The released tags were stored in a microtiter plate, which was then indexed and hybridized to barcodes. Up to 1 million data points per ROI were digitally counted and this data was analysed with nSolver™ Advanced Analysis Software (NanoString, 2018).