Exosomal lncRNA SCIRT/miR-665 Transferring Promotes Lung Cancer Cell Metastasis through the Inhibition of HEYL

Figure 2

The exosomal loading of miR-665 relies on the facilitation of SCIRT and hnRNPA1. (a) Predicted binding sites between miR-665 and lncRNA SCIRT. (b) Western blotting analysis of CD63, TSG101, and calnexin in exosomes extracted from the cell culture supernatants and lung cancer cells. RT-qPCR analysis of the expression of (c) miR-665 and (d) SCIRT in cancer-cell-derived exosomes or lung cancer cells. (e) Knockdown of SCIRT with specific siRNAs in lung cancer cells. (f) RT-qPCR analysis of miR-665 expression after SCIRT silencing. (g) RT-qPCR analysis of the expression of miR-665 and SCIRT in H446 cells after hnRNPA1 silencing. The analyses were repeated three times, and the results were expressed as mean ± SD. and .

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