Exosomal lncRNA SCIRT/miR-665 Transferring Promotes Lung Cancer Cell Metastasis through the Inhibition of HEYL

Figure 4

HEYL is the downstream target of miR-665 in lung cancer cells. (a) Schematic representation of the putative binding site of miR-665 in 3′-UTR of HEYL. (b) RT-PCR analysis of the expression of HEYL after administration of mimics or inhibitors of miR-665. (c) Dual-luciferase reporter assay was used to determine the effects of miR-665 expression on the activities of wild-type (WT) or mutant-type (MUT) HEYL 3′-UTR. (d) Transwell invasion assay of cells with or without HEYL knockdown was performed following treatment with exosomes derived from cancer cells. (e) RT-qPCR analysis of epithelial and mesenchymal markers after administration of mimics or inhibitors of miR-665. and , compared with the corresponding negative control (NC) group.

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