Y-Box Binding Protein 1 Regulates Angiogenesis in Bladder Cancer via miR-29b-3p-VEGFA Pathway

Figure 2

The expression level of YB-1 in four BC cell lines and the changes in expression of VEGFA and miR-29b-3p after knocking down YB-1 in EJ and UMUC3 cell lines. (a) The protein expression of EJ, UMUC3, SW780, and RT4 was measured by Western Blot. (b–d) The relative expression level of YB-1, VEGFA, and miR-29b-3p in CTRL-EJ and YB-1 KD-EJ cell lines was measured by RT-qPCR and Western Blot. (e–g) YB-1 was knocked down by siRNA in UMUC3 cells. Then the relative expression level of YB-1, VEGFA, and miR-29b-3p was measured by RT-qPCR and Western Blot. CTRL, control; YB-1 KD, YB-1 knockdown; NC, negative control; miR, microRNA; , , and .

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