FAM201A Promotes Cervical Cancer Progression and Metastasis through miR-1271-5p/Flotillin-1 Axis Targeting-Induced Wnt/β-Catenin Pathway

Figure 1

FAM201A was highly expressed in CC and enriched in CC cell cytoplasm, and its expression was negatively correlated with miR-1271-5p. (a) The expression of FAM201A was analyzed in the TCGA-CESC database, including CC samples () and normal samples () by GEPIA. (b, c, f, g) The expressions of FAM201A (b, f) and miR-1271-5p (c, g) in CC tissues and the adjacent normal tissues (b, c) as well as in HeLa, C33a, SiHa, ME180 and human endocervical epithelial HCerEpiC cells (f, g) were analyzed by qRT-PCR. (d) The correlation between FAM201A and miR-1271-5p in CC tissues was analyzed by Pearson’s correlation tests. (e) The expression of FAM201A in CC tissues and the adjacent normal tissues was assessed by fluorescence in situ hybridization (magnification: ×200; scale: 100 μm). (h, i) The subcellular localization of FAM201A was determined by qRT-PCR. (j, k) The expression of FAM201A in SiHa cells and ME180 cells was evaluated by fluorescent in situ hybridization (magnification: ×200; scale: 100 μm). ^##; ^###; ^# vs. HCerEpiC. CC: cervical cancer; qRT-PCR: quantitative reverse transcription polymerase chain reaction; NC: negative control; FAM201A: the family with sequence similarity 201-member A; M: miR-1271-5p mimic; MC: mimic control; GEPIA: Gene Expression Profiling Interactive Analysis; TCGA-CESC: Cancer Genome Atlas Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma; qRT-PCR: quantitative reverse transcription polymerase chain reaction.

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