FAM201A Promotes Cervical Cancer Progression and Metastasis through miR-1271-5p/Flotillin-1 Axis Targeting-Induced Wnt/β-Catenin Pathway

Figure 2

FAM201A overexpression increased CC cell viability and promoted migration by downregulating miR-1271-5p expression. (a-d) The expressions of FAM201A (a, b) and miR-1271-5p (C/D) in CC (SiHa and ME180) cells transfected with FAM201A overexpression plasmids or miR-1271-5p mimic alone or in combination were analyzed by qRT-PCR. (e, f) The viability of CC (SiHa and ME180) cells after transfection with FAM201A overexpression plasmids or miR-1271-5p mimic alone or in combination was measured by CCK-8 assay at 24-, 48-, and 72-h post-transfection. (g-i) The migration of CC (SiHa and ME180) cells after transfection with FAM201A overexpression plasmids or miR-1271-5p mimic alone or in combination was evaluated by the Transwell assay (magnification: ×250; scale: 50 μm). ⁺ or ^#; ⁺⁺ or ^{^^}or^##; ⁺⁺⁺ or ^{^^^} or ^###; ⁺ vs. NC+MC; ^{^}vs. FAM201A+MC; ^# vs. NC+miR-1271-5p M. CC: cervical cancer; qRT-PCR: quantitative reverse transcription polymerase chain reaction; CCK-8: cell counting kit-8; NC: negative control; FAM201A: the family with sequence similarity 201-member A; M: miR-1271-5p mimic; MC: mimic control.

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