FAM201A Promotes Cervical Cancer Progression and Metastasis through miR-1271-5p/Flotillin-1 Axis Targeting-Induced Wnt/β-Catenin Pathway

Figure 3

FAM201A overexpression promoted CC cell invasion and CC tumorigenesis in vivo by downregulating miR-1271-5p expression. (a-c) The invasion of CC (SiHa and ME180) cells transfected with FAM201A overexpression plasmids or miR-1271-5p mimic alone or in combination was evaluated by the Transwell assay (magnification: ×250; scale: 50 μm). (d) Pictures of subcutaneous xenografts formed by SiHa cells with stable overexpressed FAM201A, miR-1271-5p or both (e, f). The volume (e) and weight (f) of subcutaneous xenografts formed by SiHa cells with stable overexpressed FAM201A, miR-1271-5p or both were measured weekly (e) or at the fifth week after resection (f). ⁺ or ^#; ⁺⁺ or ^{^^}; ⁺⁺⁺ or ^{^^^} or ^###;⁺ vs. NC+MC; ^{^} vs. FAM201A+MC; ^# vs. NC+miR-1271-5p M. CC: cervical cancer; qRT-PCR: quantitative reverse transcription polymerase chain reaction; NC: negative control; FAM201A: the family with sequence similarity 201-member A; M: miR-1271-5p mimic; MC: mimic control.

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