FAM201A Promotes Cervical Cancer Progression and Metastasis through miR-1271-5p/Flotillin-1 Axis Targeting-Induced Wnt/β-Catenin Pathway

Figure 4

FAM201A sponged miR-1271-5p to increase the expression of FLOT1, a direct target of miR-1271-5p, in CC cells. (a) The targeting relationship between miR-1271-5p and LINC01106 was identified by Targetscan. (b, c) FLOT1 was confirmed as the target of LINC01106 by dual-luciferase reporter assay. (d-g) The expression of FLOT1 in CC (SiHa and ME180) cells after transfection with FAM201A overexpression plasmids or miR-1271-5p mimic alone or in combination was analyzed by qRT-PCR (f, g) and western blot (d, e), with GAPDH as the reference gene. ⁺; ⁺⁺ or ^##; ⁺⁺⁺ or ^{^^^} or ^###; ⁺ vs. NC+MC; ^{^} vs. FAM201A+MC; ^# vs. NC+miR-1271-5p M; ^& vs. control+ FLOT1-3’-UTR; ^§vs. miR-1271-5p+FLOT1-3’-UTR. CC: cervical cancer; qRT-PCR: quantitative reverse transcription polymerase chain reaction; NC: negative control; M: miR-1271-5p mimic; MC: mimic control; FLOT1: flotillin 1; 3’-UTR: 3’-untranslated regions.

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