FAM201A Promotes Cervical Cancer Progression and Metastasis through miR-1271-5p/Flotillin-1 Axis Targeting-Induced Wnt/β-Catenin Pathway

Figure 5

FLOT1 expression was repressed by miR-1271-5p upregulation in CC cells. (a, b, c, d) The expressions of miR-1271-5p (a, b) and FLOT1 (c, d) in CC (SiHa and ME180) cells following transfection with FLOT1 overexpression plasmids or miR-1271-5p mimic alone or in combination were analyzed by qRT-PCR. (e, f) The expression of FLOT1 in CC (SiHa and ME180) cells after transfection with FLOT1 overexpression plasmids or miR-1271-5p mimic alone or in combination was analyzed by western blot, with GAPDH as the reference gene. ⁺ or ^{^}; ^{^^}; ⁺⁺⁺ or ^{^^^} or ^###; ⁺ vs. FLOT1+MC; ^{^} vs. NC+MC; ^# vs. FLOT1+miR-1271-5p M. CC: cervical cancer; qRT-PCR: quantitative reverse transcription polymerase chain reaction; NC: negative control; M: miR-1271-5p mimic; MC: mimic control; WT: wild type; MUT: mutant type; FLOT1: flotillin 1.

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