FAM201A Promotes Cervical Cancer Progression and Metastasis through miR-1271-5p/Flotillin-1 Axis Targeting-Induced Wnt/β-Catenin Pathway

Figure 6

FLOT1 overexpression resisted miR-1271-5p upregulation-induced viability and inhibition in migration and invasion of CC cells. (a, b) The viability of CC (SiHa and ME180) cells transfected with FLOT1 overexpression plasmids or miR-1271-5p mimic alone or in combination was measured by CCK-8 assay at 24-, 48-, and 72-h posttransfection. (c-h). The migration (c-e) and invasion (f-h) of CC (SiHa and ME180) cells after transfection with FLOT1 overexpression plasmids or miR-1271-5p mimic alone or in combination were evaluated by the Transwell assay (magnification: ×250; scale: 50 μm). ⁺ or ^{^} or ^#; ⁺⁺ or ^{^^} or ^##; ⁺⁺⁺ or ^{^^^} or ^###; ⁺ vs. FLOT1+MC; ^{^} vs. NC+MC; ^# vs. FLOT1+miR-1271-5p M. CC: cervical cancer; qRT-PCR: quantitative reverse transcription polymerase chain reaction; CCK-8: cell counting kit-8; NC: negative control; M: miR-1271-5p mimic; MC: mimic control; FLOT1: flotillin 1.

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