FAM201A Promotes Cervical Cancer Progression and Metastasis through miR-1271-5p/Flotillin-1 Axis Targeting-Induced Wnt/β-Catenin Pathway

Figure 8

FLOT1 overexpression resisted miR-1271-5p upregulation-induced suppression on the Wnt/β-catenin signaling pathway in CC cells. (a-h). The expressions of Wnt1, β-catenin, p-β-catenin and p-β-catenin/β-catenin in CC (SiHa and ME180) cells transfected with FLOT1 overexpression plasmids or miR-1271-5p mimic alone or in combination were determined by qRT-PCR (G/H) and western blot (a-f), with GAPDH as the reference gene. ⁺; ⁺⁺ or ^{^^} or ^##; ⁺⁺⁺ or ^{^^^} or ^###; ⁺ vs. FLOT1+MC; ^{^} vs. NC+MC; ^# vs. FLOT1+miR-1271-5p M. CC: cervical cancer; qRT-PCR: quantitative reverse transcription polymerase chain reaction; NC: negative control; M: miR-1271-5p mimic; MC: mimic control; FLOT1: flotillin 1.

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