Ai-Tong-An-Gao-Ji and Fisetin Inhibit Tumor Cell Growth in Rat CIBP Models by Inhibiting the AKT/HIF-1α Signaling Pathway

Figure 4

Fisetin affects tumor cell proliferation; (a) colony formation assay of each group of cells; (b) quantification of several colonies in Figure 4(a); (c) transwell experiment of cells in each group; (d) quantification of several cells in Figure 4(c); (e) EDU experiment of each group; (f) quantification of EDU⁺ cells in Figure 4(e). We divided the Walker 256 cells into a control group, cisplatin group, low dose fisetin group, medium dose fisetin group, and a high dose fisetin group. Walker 256 cells were treated with fisetin (10 μM, 20 μM, and 30 μM) and cisplatin (5 μM) in the positive control group for 24 h Student’s t-tests (two groups) or one-way ANOVA was employed and followed by Tukey’s tests (more than two groups) (n ≥ 3). , , .

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