Research Article
GNL3 Regulates SIRT1 Transcription and Promotes Hepatocellular Carcinoma Stem Cell-Like Features and Metastasis
Figure 2
The effect of GNL3 on the growth and migration of liver cancer cells. (a) qRT-PCR was used to detect transfection efficiency 48 h after transfection. (b) CCK-8 assay to evaluate cell viability after transfection. (c) Colony formation test to detect the clone formation ability of HepG2 and PLC-PRF-5 cells. The transfected liver cancer cells were cultured in medium containing 10% fetal bovine serum for 2 weeks. (d) The transwell method to evaluate the invasion ability of HepG2 and PLC-PRF-5 cells. and .
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