Research Article

GNL3 Regulates SIRT1 Transcription and Promotes Hepatocellular Carcinoma Stem Cell-Like Features and Metastasis

Figure 4

GNL3 upregulates the expression of SIRT1. (a) After overexpression of GNL3, the expression of SIRT1 in the transfected liver cancer cells was detected by qRT-PCR. (b) Collected liver cancer tumor tissue and adjacent nontumor tissue samples were matched from 20 liver cancer patients. The qRT-PCR method was used to detect the level of SIRT1 mRNA in tissue samples. (c) Immunohistochemical detection of SIRT1 expression in human liver cancer tumor tissue and adjacent nontumor tissue specimens. (d) Pearson’s correlation coefficient was used to analyse the relationship between GNL3 and SIRT1. (e) Immunofluorescence to detect the colocalization of GNL3 and SIRT1. .
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