B-Cell Translocation Gene 2 Upregulation Is Associated with Favorable Prognosis in Lung Adenocarcinoma and Prolonged Patient Survival

Figure 3

(a) The Western blot of BTG2 expression in selected clones of parental and BTG2 overexpressed cells. BTG2 expression level in HeLa cells served as a positive control and β-actin level as an internal control. (b) Cell growth determined by CCK-8 assays in BTG2- or vector-transfected A549 cells. (c) Representative cell cycle and flow cytometry data. (d) Cellular apoptosis was assessed via flow cytometry analysis using Annexin V-FITC and PI double staining. Data are presented as the and represent three independent experiments. by Student’s -test.

(a)

(b)

(c)

(d)